Hotspot

ndhE-psaC

SSC · 124041-124793 · 753 bp

Back to Nervilia

Pi 0.214

MarkerSeek score 84.30

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 753
variable_sites: 153
pi: 0.214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	GTCGAAGCATGGTTAGAGCA	GGACAAGCGGATTCACATCT	50.0	50.0	58.0	58.0	1165-1483 bp	1.0	90.0
ndhE-psaC_p2	TCGAAGCATGGTTAGAGCAC	GGACAAGCGGATTCACATCT	50.0	50.0	58.0	58.0	1164-1482 bp	1.0	90.0
ndhE-psaC_p3	CGAAGCATGGTTAGAGCACT	GGACAAGCGGATTCACATCT	50.0	50.0	58.0	58.0	1163-1481 bp	1.0	89.9
ndhE-psaC_p4	GTCGAAGCATGGTTAGAGCA	TGCAACCTACACAATCCTCG	50.0	50.0	58.0	57.9	1143-1461 bp	1.0	89.5
ndhE-psaC_p5	TCGAAGCATGGTTAGAGCAC	TGCAACCTACACAATCCTCG	50.0	50.0	58.0	57.9	1142-1460 bp	1.0	89.5