Hotspot

psbB-psbT

LSC · 76473-77020 · 548 bp

Back to Nervilia

Pi 0.284

MarkerSeek score 79.90

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 153
pi: 0.284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TGTACGTCGTATGCCTACCT	CAACCCTAGTCGCCATCTTC	50.0	55.0	58.0	58.1	1304-1559 bp	1.0	87.7
psbB-psbT_p2	ATTTGGCCCCACTCGTTATC	CAACCCTAGTCGCCATCTTC	50.0	55.0	57.9	58.1	1568-1823 bp	1.0	87.5
psbB-psbT_p3	GAGATAAAGAAGGGCGCGAA	CAACCCTAGTCGCCATCTTC	50.0	55.0	58.1	58.1	1329-1584 bp	1.0	87.5
psbB-psbT_p4	TATGGTGGCGAACTCAATGG	CAACCCTAGTCGCCATCTTC	50.0	55.0	58.0	58.1	1165 bp	0.5	59.2
psbB-psbT_p5	TTTTTCGTAGCAGTCCGAGG	CAACCCTAGTCGCCATCTTC	50.0	55.0	57.9	58.1	1044 bp	0.5	58.9