Hotspot

psbE-petL

LSC · 66421-67862 · 1442 bp

Back to Nervilia

Pi 0.102

MarkerSeek score 84.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1442
variable_sites: 138
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.9	57.9	2091-2098 bp	1.0	88.7
psbE-petL_p2	ATGGAATTCCTTGTCGGCTC	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.0	57.9	2180-2187 bp	1.0	88.5
psbE-petL_p3	ATGGAATTCCTTGTCGGCTC	ACCGCGCCTGTATTGTAAAT	50.0	45.0	58.0	58.0	2010 bp	0.5	59.4
psbE-petL_p4	CAACCCGCAATGAATAGGGA	ACCGCGCCTGTATTGTAAAT	50.0	45.0	57.9	58.0	1921 bp	0.5	59.3
psbE-petL_p5	ACTGACGAATAACCAACCCG	ACCGCGCCTGTATTGTAAAT	50.0	45.0	57.9	58.0	1934 bp	0.5	59.0