Hotspot

petA-psbJ

LSC · 64749-65640 · 892 bp

Back to Nervilia

Pi 0.0855

MarkerSeek score 82.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 71
pi: 0.0855
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGGTTCAGTTGTACGAAATGA	GGGTTGGGTTCATCTCTGTA	38.1	50.0	55.2	56.5	942-1032 bp	1.0	49.8
petA-psbJ_p2	TGAAAAGGTTCAGTTGTACGA	GGGTTGGGTTCATCTCTGTA	38.1	50.0	55.4	56.5	947-1037 bp	1.0	49.7
petA-psbJ_p3	ACAGTTTGAAAAGGTTCAGTTGT	GGGTTGGGTTCATCTCTGTA	34.8	50.0	57.3	56.5	953-1043 bp	1.0	49.7
petA-psbJ_p4	TGAAAAGGTTCAGTTGTACGAA	GGGTTGGGTTCATCTCTGTA	36.4	50.0	56.2	56.5	947-1037 bp	1.0	49.7
petA-psbJ_p5	TTGAAAAGGTTCAGTTGTACGA	GGGTTGGGTTCATCTCTGTA	36.4	50.0	56.2	56.5	948-1038 bp	1.0	49.7