Hotspot

petN-psbM

LSC · 29329-30221 · 893 bp

Back to Nervilia

Pi 0.174

MarkerSeek score 80.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 893
variable_sites: 132
pi: 0.174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATCTGGGTTCTGAGTGTGGA	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.0	57.9	1718 bp	0.5	59.1
petN-psbM_p2	TCTGGGTTCTGAGTGTGGAT	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.0	57.9	1717 bp	0.5	59.1
petN-psbM_p3	AGTATGGGGAAGGAGTGGTC	CGTTTATTGCTACTGCGCTG	55.0	50.0	58.1	57.9	1004 bp	0.5	58.4
petN-psbM_p4	GTATGGGGAAGGAGTGGTCT	CGTTTATTGCTACTGCGCTG	55.0	50.0	58.1	57.9	1003 bp	0.5	58.4
petN-psbM_p5	TCTGGGTTCTGAGTGTGGAT	TAAAAGCGGAAAGGCGGTAA	50.0	45.0	58.0	57.8	2016 bp	0.5	58.3