Hotspot

rpoB-trnC-GCA

LSC · 27330-28357 · 1028 bp

Back to Nervilia

Pi 0.17

MarkerSeek score 85.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 165
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCTGGA	GCGACACCCAGATTTGAACT	45.0	50.0	55.8	58.5	1121-1432 bp	1.0	70.1
rpoB-trnC-GCA_p2	TCCCTCATTTCCATTCTGGA	TAAAGGATTTGCAGTCCCCC	45.0	50.0	55.8	57.5	1096-1407 bp	1.0	69.8
rpoB-trnC-GCA_p3	TTCCCTCATTTCCATTCTGGA	GCGACACCCAGATTTGAACT	42.9	50.0	56.6	58.5	1122-1433 bp	1.0	68.5
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCTGGA	TAAAGGATTTGCAGTCCCCC	42.9	50.0	56.6	57.5	1097-1408 bp	1.0	68.2
rpoB-trnC-GCA_p5	TCCCTCATTTCCATTCTGGA	TTTGTTGATCAGGCGACACC	45.0	50.0	55.8	58.8	1133-1444 bp	1.0	67.8