Hotspot

trnS-GCU-trnG-UCC

LSC · 8538-9610 · 1073 bp

Back to Nervilia

Pi 0.151

MarkerSeek score 76.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1073
variable_sites: 113
pi: 0.151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CCGAGCTGAGACAAGAAGAC	50.0	55.0	58.0	58.0	1280-1535 bp	1.0	91.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	CCGAGCTGAGACAAGAAGAC	50.0	55.0	58.0	58.0	1279-1534 bp	1.0	91.7
trnS-GCU-trnG-UCC_p3	ATCTAATGATCCGGGGCGTA	CCGAGCTGAGACAAGAAGAC	50.0	55.0	58.1	58.0	1524-1788 bp	1.0	90.7
trnS-GCU-trnG-UCC_p4	TATCTAATGATCCGGGGCGT	CCGAGCTGAGACAAGAAGAC	50.0	55.0	58.1	58.0	1525-1789 bp	1.0	90.7
trnS-GCU-trnG-UCC_p5	ATCGGAGAGAGAGGGATTCG	CCGAGCTGAGACAAGAAGAC	55.0	55.0	58.1	58.0	1335-1590 bp	1.0	90.7