Hotspot

psbK-psbI

LSC · 7748-8178 · 431 bp

Back to Nervilia

Pi 0.145
MarkerSeek score 81.90
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
431
variable_sites
60
pi
0.145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACTACTGTGTAAACGAAGAGT 50.0 38.1 57.9 54.3 496-569 bp 1.0 52.5
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG ACGAAGAGTTTAAGAGTAAGCA 50.0 36.4 57.9 55.2 484-557 bp 1.0 51.6
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG ACTACTGTGTAAACGAAGAGTT 50.0 36.4 57.9 55.1 496-569 bp 1.0 51.0
psbK-psbI_p4 TGGCAAGCTGCTGTAAGTTT ACTACTGTGTAAACGAAGAGT 45.0 38.1 58.3 54.3 493-566 bp 1.0 50.6
psbK-psbI_p5 TTGGCAAGCTGCTGTAAGTT ACTACTGTGTAAACGAAGAGT 45.0 38.1 58.3 54.3 494-567 bp 1.0 50.6