Hotspot

rps16-trnQ-UUG

LSC · 6279-7137 · 859 bp

Back to Nervilia

Pi 0.228
MarkerSeek score 85.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
859
variable_sites
167
pi
0.228
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 919-932 bp 1.0 90.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 923-936 bp 1.0 89.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TACTCGGAGGTTCGAATCCT 45.0 50.0 58.1 57.6 925-938 bp 1.0 87.4
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA ACTCGGAGGTTCGAATCCTT 45.0 50.0 58.1 58.4 924-937 bp 1.0 87.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAGACCAAAAGAATGGGGCG 45.0 50.0 58.1 57.9 993 bp 0.5 57.9