Hotspot

trnK-UUU-rps16

LSC · 4593-5146 · 554 bp

Back to Nervilia

Pi 0.106

MarkerSeek score 82.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 55
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CTTTTCCCACATCAGGCACT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	1168-1193 bp	1.0	88.0
trnK-UUU-rps16_p2	ACCATATACCCCGGAGACAG	TCGATCCCGAAGAGAAGGAA	55.0	50.0	58.0	57.9	1464 bp	0.5	58.8
trnK-UUU-rps16_p3	ACCATATACCCCGGAGACAG	TCGTTGCAATTGATGGTCGA	55.0	45.0	58.0	58.2	1480 bp	0.5	58.3
trnK-UUU-rps16_p4	ACCATATACCCCGGAGACAG	CCTTGAAAAAGGCGCTCAAC	55.0	50.0	58.0	58.2	1368 bp	0.5	58.1
trnK-UUU-rps16_p5	ACCATATACCCCGGAGACAG	CTTGAAAAAGGCGCTCAACC	55.0	50.0	58.0	58.2	1367 bp	0.5	58.1