Hotspot

matK-trnK-UUU

LSC · 3343-4555 · 1213 bp

Back to Nervilia

Pi 0.153

MarkerSeek score 84.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1213
variable_sites: 171
pi: 0.153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GGATGGGGTGGGGTATTAGT	TACGGGTTGCTAACTCAACG	55.0	50.0	58.2	57.9	1737-1826 bp	1.0	90.1
matK-trnK-UUU_p2	TGGATGGGGTGGGGTATTAG	TACGGGTTGCTAACTCAACG	55.0	50.0	58.2	57.9	1738-1827 bp	1.0	90.1
matK-trnK-UUU_p3	GGATGGGGTGGGGTATTAGT	AACGGTAGAGTACTCGGCTT	55.0	50.0	58.2	58.2	1721-1810 bp	1.0	89.3
matK-trnK-UUU_p4	GGATGGGGTGGGGTATTAGT	ACGGTAGAGTACTCGGCTTT	55.0	50.0	58.2	58.2	1720-1809 bp	1.0	89.3
matK-trnK-UUU_p5	CATATGGATGGGGTGGGGTA	TACGGGTTGCTAACTCAACG	55.0	50.0	58.2	57.9	1742 bp	0.5	57.5