Hotspot

ndhF

SSC · 112656-114881 · 2226 bp

Pi 0.129

MarkerSeek score 79.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 948
pi: 0.129
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGATCCACCGAACAAGACAA	TTTGTTCCTTTCGAAAGCGA	50.0	40.0	57.8	56.2	2445-2505 bp	0.75	57.0
ndhF_p2	ACCAAGTTCAACGTTAGCCA	TTTGTTCCTTTCGAAAGCGA	45.0	40.0	57.7	56.2	2821-2890 bp	0.75	55.4
ndhF_p3	ACAGGAACAAGAGGGATCCA	TTTGTTCCTTTCGAAAGCGA	50.0	40.0	58.0	56.2	2458-2518 bp	0.5	47.5
ndhF_p4	ACAGGAACAAGAGGGATCCA	TTTTGTTCCTTTCGAAAGCGA	50.0	38.1	58.0	57.0	2459-2519 bp	0.5	45.5
ndhF_p5	ACAGGAACAAGAGGGATCCA	GCGATAAGAATTGGTGAATCGG	50.0	45.5	58.0	57.9	2442-2502 bp	0.5	44.7