Hotspot

rps15-ycf1

SSC · 126281-126653 · 373 bp

Back to Nerine

Pi 0.0448
MarkerSeek score 70.40
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
373
variable_sites
43
pi
0.0448
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AACACCATGCATTGATGGGT ATCTCCCAGACGTCGTAGAG 45.0 55.0 58.1 58.0 1135-1158 bp 1.0 85.2
rps15-ycf1_p2 CCACCGTGTTACATAAGGCA ATCTCCCAGACGTCGTAGAG 50.0 55.0 57.9 58.0 1270-1293 bp 1.0 84.3
rps15-ycf1_p3 TCCCGAATACCCAACTGACT ATCTCCCAGACGTCGTAGAG 50.0 55.0 58.1 58.0 931-941 bp 0.875 78.3
rps15-ycf1_p4 TCCCGAATACCCAACTGACT GGAGTGCACCCTTGAATCAA 50.0 50.0 58.1 58.1 1340-1350 bp 0.625 66.6
rps15-ycf1_p5 AACACCATGCATTGATGGGT GGAGTGCACCCTTGAATCAA 45.0 50.0 58.1 58.1 1557-1567 bp 0.625 66.4