Hotspot

rpl32-trnL-UAG

SSC · 115990-116717 · 728 bp

Back to Nerine

Pi 0.036
MarkerSeek score 71.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
728
variable_sites
67
pi
0.036
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTTTGTGCGACAAACAAGT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 55.2 57.9 789-867 bp 1.0 64.3
rpl32-trnL-UAG_p2 TTTTTGTGCGACAAACAAGT AGCAGCGTGTCTACCAATTT 35.0 45.0 55.2 57.8 782-860 bp 1.0 63.9
rpl32-trnL-UAG_p3 TTTTTGTGCGACAAACAAGT CCTAAGAGCAGCGTGTCTAC 35.0 55.0 55.2 57.8 788-866 bp 1.0 63.8
rpl32-trnL-UAG_p4 TTTTTGTGCGACAAACAAGT CTAAGAGCAGCGTGTCTACC 35.0 55.0 55.2 57.8 787-865 bp 1.0 63.8
rpl32-trnL-UAG_p5 TTTTTGTGCGACAAACAAGT GAGCAGCGTGTCTACCAATT 35.0 50.0 55.2 58.3 783-861 bp 1.0 63.2