Hotspot

trnH-GUG-rpl2

IRb · 86543-86589 · 47 bp

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Pi 0.0418
MarkerSeek score 66.80
Primer available yes
Label trnH-GUG-rpl2

Feature Metadata

label_name
trnH-GUG-rpl2
feature_type
igs
region
IRb
length_bp
47
variable_sites
5
pi
0.0418
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-rpl2_p1 TGGATCAAGGCAGTGGATTG GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 183 bp 1.0 80.9
trnH-GUG-rpl2_p2 GGATCAAGGCAGTGGATTGT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 182 bp 1.0 80.9
trnH-GUG-rpl2_p3 GTGGATCAAGGCAGTGGATT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 184 bp 1.0 80.9
trnH-GUG-rpl2_p4 CGGATGTAGCCAAGTGGATC GTTATCCTGCGCTTGGAAGA 55.0 50.0 58.1 58.0 197 bp 1.0 80.8
trnH-GUG-rpl2_p5 CGGATGTAGCCAAGTGGATC TATCCTGCGCTTGGAAGAAG 55.0 50.0 58.1 57.7 195 bp 1.0 78.5