Hotspot

rpl22-rps19

IRb · 86005-86056 · 52 bp

Back to Nerine

Pi 0.0474
MarkerSeek score 67.40
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
52
variable_sites
7
pi
0.0474
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TGTTTCCTCATAAGAGCGCC TCTGACTTTCGCGAGACATG 50.0 50.0 58.0 58.0 223-238 bp 1.0 88.1
rpl22-rps19_p2 TGTTTCCTCATAAGAGCGCC GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 788-799 bp 1.0 83.0
rpl22-rps19_p3 TCGAAGCCCGAGGATATTCT TCTGACTTTCGCGAGACATG 50.0 50.0 58.0 58.0 714-732 bp 0.75 74.7
rpl22-rps19_p4 TCGAAGCCCGAGGATATTCT TAGTAACTTGGTCTCGGGCA 50.0 50.0 58.0 58.1 853-871 bp 0.75 73.6
rpl22-rps19_p5 TCGAAGCCCGAGGATATTCT GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 1275-1299 bp 0.75 73.1