Hotspot

atpB-rbcL

LSC · 56406-57317 · 912 bp

Back to Nerine

Pi 0.0339
MarkerSeek score 70.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
912
variable_sites
71
pi
0.0339
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GCCGACTGGAACACTTAGAG GTCCACACAGTTGTCCATGT 55.0 50.0 58.0 58.0 1393-1455 bp 1.0 88.0
atpB-rbcL_p2 GCCGACTGGAACACTTAGAG AGATCCTCCAGACGTAGAGC 55.0 55.0 58.0 58.0 1594-1656 bp 1.0 87.4
atpB-rbcL_p3 GCCGACTGGAACACTTAGAG ATCCTCCAGACGTAGAGCTC 55.0 55.0 58.0 58.0 1592-1654 bp 1.0 87.4
atpB-rbcL_p4 GCCGACTGGAACACTTAGAG GATCCTCCAGACGTAGAGCT 55.0 55.0 58.0 58.0 1593-1655 bp 1.0 87.4
atpB-rbcL_p5 GCCGACTGGAACACTTAGAG CGGTCTCTCCAACGCATAAA 55.0 50.0 58.0 58.0 1831-1893 bp 1.0 86.6