Hotspot

trnC-GCA-petN

LSC · 28419-29495 · 1077 bp

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Pi 0.0686
MarkerSeek score 85.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1077
variable_sites
276
pi
0.0686
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GGGGGACTGCAAATCCTTTA TTAAAGCAGCCCAAGCAAGA 50.0 45.0 57.5 58.0 1156-1163 bp 1.0 87.8
trnC-GCA-petN_p2 TTTTGGATCGAATTTGGCGG TTAAAGCAGCCCAAGCAAGA 45.0 45.0 57.4 58.0 1195-1202 bp 1.0 86.8
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTA CAGCCCAAGCAAGACTTACT 50.0 50.0 57.5 57.8 1150-1157 bp 1.0 86.3
trnC-GCA-petN_p4 TTTTGGATCGAATTTGGCGG CAGCCCAAGCAAGACTTACT 45.0 50.0 57.4 57.8 1189-1196 bp 1.0 85.3
trnC-GCA-petN_p5 CCAGTTCAAATCCGGGTGTC TTAAAGCAGCCCAAGCAAGA 55.0 45.0 58.8 58.0 1133-1140 bp 1.0 85.3