Hotspot

rpl32-ndhF

SSC · 134649-135669 · 1021 bp

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Pi 0.00615

MarkerSeek score 66.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1021
variable_sites: 6
pi: 0.00615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AGAAAGGGATTTCGCTAACG	GCGGGAAAAAGGAGAAGTCC	45.0	55.0	55.9	58.8	1188-1230 bp	1.0	59.7
rpl32-ndhF_p2	AGAAAGGGATTTCGCTAACG	CTGGAAGTGGAATGAAGGGT	45.0	50.0	55.9	57.1	1148-1190 bp	1.0	59.3
rpl32-ndhF_p3	AGAAAGGGATTTCGCTAACG	CGCGGGAAAAAGGAGAAGT	45.0	52.6	55.9	58.1	1189-1231 bp	1.0	57.7
rpl32-ndhF_p4	AAAGCTTTCAATGCTACCCA	GCGGGAAAAAGGAGAAGTCC	40.0	55.0	55.5	58.8	1166-1208 bp	1.0	57.0
rpl32-ndhF_p5	AAGCTTTCAATGCTACCCAA	GCGGGAAAAAGGAGAAGTCC	40.0	55.0	55.5	58.8	1165-1207 bp	1.0	57.0