Hotspot

ndhJ-ndhK

LSC · 56173-56342 · 170 bp

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Pi 0.0203

MarkerSeek score 66.40

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 3
pi: 0.0203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CAAGTAGACAACCGACCCTG	AGTTCAGTATCCTCCCACGA	55.0	50.0	57.9	57.5	204-226 bp	1.0	80.3
ndhJ-ndhK_p2	CAAGTAGACAACCGACCCTG	CAGAGATACCCGCTGAAACA	55.0	50.0	57.9	57.3	239-261 bp	1.0	78.9
ndhJ-ndhK_p3	ATCGAATGCCAATCCTCAGG	AGTTCAGTATCCTCCCACGA	50.0	50.0	57.7	57.5	297-319 bp	1.0	78.4
ndhJ-ndhK_p4	GCAATCGAATGCCAATCCTC	AGTTCAGTATCCTCCCACGA	50.0	50.0	57.6	57.5	300-322 bp	1.0	77.3
ndhJ-ndhK_p5	ATCGAATGCCAATCCTCAGG	CAGAGATACCCGCTGAAACA	50.0	50.0	57.7	57.3	332-354 bp	1.0	77.2