Hotspot

trnS-GCU-trnG-UCC

LSC · 9681-11324 · 1644 bp

Back to Neptunia

Pi 0.0209

MarkerSeek score 66.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1644
variable_sites: 31
pi: 0.0209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1608-1756 bp	1.0	79.8
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1607-1755 bp	1.0	79.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.2	57.3	1662-1810 bp	1.0	78.5
trnS-GCU-trnG-UCC_p4	TGGAAACGGAAAGAGAGGGA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.3	57.3	1667-1815 bp	1.0	77.5
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	1576-1724 bp	1.0	77.3