Hotspot

ndhF

SSC · 105233-107476 · 2244 bp

Back to Nephrolepis

Pi 0.0659

MarkerSeek score 73.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 147
pi: 0.0659
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCGAGGTAGAGATCAACCCC	ACAGCTGCATCATTCAACCA	55.0	45.0	57.9	58.1	2981 bp	1.0	84.3
ndhF_p2	TCGAGGTAGAGATCAACCCC	TATTGAACTGGGGCGAAGTG	55.0	50.0	57.9	57.9	2879 bp	1.0	84.2
ndhF_p3	TCGAGGTAGAGATCAACCCC	GTATTGAACTGGGGCGAAGT	55.0	50.0	57.9	57.9	2880 bp	1.0	84.2
ndhF_p4	CGAGGTAGAGATCAACCCCA	ACAGCTGCATCATTCAACCA	55.0	45.0	58.2	58.1	2980 bp	1.0	83.1
ndhF_p5	TCGAGGTAGAGATCAACCCC	TGCATCATTCAACCACGGAT	55.0	45.0	57.9	57.9	2976 bp	0.5	58.4