Hotspot

ndhB

LSC · 1-1198 · 1198 bp

Back to Nephrolepis

Pi 0.0939

MarkerSeek score 79.70

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: LSC
length_bp: 1198
variable_sites: 111
pi: 0.0939
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	GCTTATGAAGGATCAAGCCCA	TTGATGGGTGGAGCAAGTTC	47.6	50.0	58.1	58.1	607-642 bp	1.0	79.2
ndhB_p2	GCTTATGAAGGATCAAGCCCA	TTGGTCACCCTTTATGTGGC	47.6	50.0	58.1	58.1	715-750 bp	1.0	78.4
ndhB_p3	GCTTATGAAGGATCAAGCCCA	GGTTTAGCCGGAATGGTTCT	47.6	50.0	58.1	57.9	751-786 bp	1.0	77.9
ndhB_p4	GCTTATGAAGGATCAAGCCCA	CTATATGGAACGTCTGGCGG	47.6	55.0	58.1	57.9	559 bp	0.5	51.0
ndhB_p5	GCTTATGAAGGATCAAGCCCA	GGGGACACAAAAACTTCCCT	47.6	50.0	58.1	57.9	301 bp	0.5	50.8