Hotspot

matK

LSC · 1417-2910 · 1494 bp

Back to Nephrolepis

Pi 0.0877

MarkerSeek score 77.90

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1494
variable_sites: 131
pi: 0.0877
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AGAACCATTCCGGCTAAACC	TCAACAGCAACAGTTCGTGA	50.0	45.0	57.9	58.0	2826-2853 bp	1.0	87.4
matK_p2	GACCAAATCATTTGCTCGGC	TCAACAGCAACAGTTCGTGA	50.0	45.0	58.1	58.0	2875 bp	0.5	59.2
matK_p3	GACCAAATCATTTGCTCGGC	TCGGAGGGACTACTGGTTTT	50.0	50.0	58.1	58.0	2672 bp	0.5	59.1
matK_p4	GACCAAATCATTTGCTCGGC	TTCGGAGGGACTACTGGTTT	50.0	50.0	58.1	58.0	2673 bp	0.5	59.1
matK_p5	GACCAAATCATTTGCTCGGC	TTTCGGAGGGACTACTGGTT	50.0	50.0	58.1	58.0	2674 bp	0.5	59.1