Hotspot

trnL-UAG-ccsA

SSC · 109534-109815 · 282 bp

Back to Nephrolepis

Pi 0.135

MarkerSeek score 72.90

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 282
variable_sites: 38
pi: 0.135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	CTCTTAGGAAGCAGTGCCAG	AAGATCCGCACAACAAGGTT	55.0	45.0	58.0	58.0	790-806 bp	1.0	85.9
trnL-UAG-ccsA_p2	CTTAGGAAGCAGTGCCAGAG	AAAGATCCGCACAACAAGGT	55.0	45.0	58.0	58.0	789-805 bp	1.0	85.9
trnL-UAG-ccsA_p3	CTTAGGAAGCAGTGCCAGAG	AAGATCCGCACAACAAGGTT	55.0	45.0	58.0	58.0	788-804 bp	1.0	85.9
trnL-UAG-ccsA_p4	CTCTTAGGAAGCAGTGCCAG	GCAGGTACTAATGGCGTCAA	55.0	50.0	58.0	58.0	716 bp	0.5	59.7
trnL-UAG-ccsA_p5	CTTAGGAAGCAGTGCCAGAG	GCAGGTACTAATGGCGTCAA	55.0	50.0	58.0	58.0	714 bp	0.5	59.7