Hotspot

trnP-GGG-trnL-UAG

SSC · 109156-109454 · 299 bp

Back to Nephrolepis

Pi 0.154
MarkerSeek score 78.70
Primer available yes
Label trnP-GGG-trnL-UAG

Feature Metadata

label_name
trnP-GGG-trnL-UAG
feature_type
igs
region
SSC
length_bp
299
variable_sites
40
pi
0.154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-trnL-UAG_p1 GCTACCAAACTGCGCCATAT CTCTGGCACTGCTTCCTAAG 50.0 55.0 58.7 58.0 336-376 bp 1.0 86.3
trnP-GGG-trnL-UAG_p2 GCTACCAAACTGCGCCATAT CTGGCACTGCTTCCTAAGAG 50.0 55.0 58.7 58.0 334-374 bp 1.0 86.3
trnP-GGG-trnL-UAG_p3 GACCTCCATCACCCCAAGAT CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.8 58.0 361-401 bp 1.0 85.6
trnP-GGG-trnL-UAG_p4 GACCTCCATCACCCCAAGAT CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.8 58.0 359-399 bp 1.0 85.6
trnP-GGG-trnL-UAG_p5 GCTACCAAACTGCGCCATAT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.7 57.9 323-363 bp 1.0 85.5