Hotspot

petG-trnW-CCA

LSC · 62337-62604 · 268 bp

Back to Nephrolepis

Pi 0.116
MarkerSeek score 80.90
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
268
variable_sites
30
pi
0.116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 CCACAATATCGACGTGGTGA CAAAACCCGATGTCGTAGGT 50.0 50.0 57.7 57.9 343-346 bp 1.0 86.0
petG-trnW-CCA_p2 ATCGGGAATTGTGTTGGGTT CAAAACCCGATGTCGTAGGT 45.0 50.0 57.7 57.9 404-407 bp 1.0 84.6
petG-trnW-CCA_p3 TGTGTTGGGTTCGATTCCAA CAAAACCCGATGTCGTAGGT 45.0 50.0 57.6 57.9 395-398 bp 1.0 84.4
petG-trnW-CCA_p4 TTGTGTTGGGTTCGATTCCA CAAAACCCGATGTCGTAGGT 45.0 50.0 57.6 57.9 396-399 bp 1.0 84.3
petG-trnW-CCA_p5 GGGAATTGTGTTGGGTTCGA CAAAACCCGATGTCGTAGGT 50.0 50.0 58.4 57.9 401-404 bp 1.0 83.9