Hotspot

petA-psbJ

LSC · 59687-60420 · 734 bp

Back to Nephrolepis

Pi 0.104

MarkerSeek score 83.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 72
pi: 0.104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCTGCGTATTCAAGGTCTCA	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	885-927 bp	1.0	90.0
petA-psbJ_p2	GCCGATCAGCCATTGACTAA	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	962-1004 bp	1.0	89.0
petA-psbJ_p3	GCTGCGTATTCAAGGTCTCA	GATGGTTAGCTGTTCACGGT	50.0	50.0	58.0	57.9	1274-1319 bp	1.0	87.2
petA-psbJ_p4	ACCGTCCTGGCAAAGAAAAT	GCCGGTACACTTGTGATAGG	45.0	55.0	58.0	58.1	1318-1360 bp	1.0	87.1
petA-psbJ_p5	ACCGTCCTGGCAAAGAAAAT	GATGGTTAGCTGTTCACGGT	45.0	50.0	58.0	57.9	1707-1752 bp	1.0	85.6