Hotspot

trnL-UAA-trnF-GAA

LSC · 45895-46215 · 321 bp

Back to Nephrolepis

Pi 0.119
MarkerSeek score 78.40
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
321
variable_sites
37
pi
0.119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 GGGTTCGACTCCCTCTATCC TTTGAACTGGTGACACGAGG 60.0 50.0 58.7 58.1 393-403 bp 1.0 83.7
trnL-UAA-trnF-GAA_p2 GGTTCGACTCCCTCTATCCC TTTGAACTGGTGACACGAGG 60.0 50.0 58.7 58.1 392-402 bp 1.0 83.7
trnL-UAA-trnF-GAA_p3 GTTCGACTCCCTCTATCCCC TTTGAACTGGTGACACGAGG 60.0 50.0 58.7 58.1 391-401 bp 1.0 83.7
trnL-UAA-trnF-GAA_p4 TTCGACTCCCTCTATCCCCA TTTGAACTGGTGACACGAGG 55.0 50.0 59.1 58.1 390-400 bp 1.0 80.6
trnL-UAA-trnF-GAA_p5 GTTCGACTCCCTCTATCCCC GAACTGGTGACACGAGGATT 60.0 50.0 58.7 57.5 388-398 bp 1.0 80.6