Hotspot

trnT-GGU-trnfM-CAU

LSC · 35327-35790 · 464 bp

Back to Nephrolepis

Pi 0.0945
MarkerSeek score 83.80
Primer available yes
Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name
trnT-GGU-trnfM-CAU
feature_type
igs
region
LSC
length_bp
464
variable_sites
43
pi
0.0945
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnfM-CAU_p1 TTAGTTAGCCCCTTGTCGGA GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 612-619 bp 1.0 86.1
trnT-GGU-trnfM-CAU_p2 TTAGCCCCTTGTCGGATTTG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 608-615 bp 1.0 85.6
trnT-GGU-trnfM-CAU_p3 GTTAGCCCCTTGTCGGATTT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 609-616 bp 1.0 85.6
trnT-GGU-trnfM-CAU_p4 TAGTTAGCCCCTTGTCGGAT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 611-618 bp 1.0 85.2
trnT-GGU-trnfM-CAU_p5 TTAGTTAGCCCCTTGTCGGA TAACTAAGCGGGGTAGAGCA 50.0 50.0 58.1 57.9 622-629 bp 1.0 85.0