Hotspot

psbL

LSC · 66910-67026 · 117 bp

Back to Nephelium

Pi 0.0171

MarkerSeek score 62.30

Primer available yes

Label psbL

Feature Metadata

label_name: psbL
feature_type: gene
region: LSC
length_bp: 117
variable_sites: 2
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL_p1	TGTGATCCTTCCGAATGGGA	ACGGACTAGCTGTACCTACC	50.0	55.0	58.4	58.0	278 bp	1.0	76.8
psbL_p2	TGTGATCCTTCCGAATGGGA	GGACTAGCTGTACCTACCGT	50.0	55.0	58.4	58.0	276 bp	1.0	76.8
psbL_p3	CCTTCCGAATGGGATAAGCG	ACGGACTAGCTGTACCTACC	55.0	55.0	58.5	58.0	272 bp	1.0	76.3
psbL_p4	CCTTCCGAATGGGATAAGCG	GGACTAGCTGTACCTACCGT	55.0	55.0	58.5	58.0	270 bp	1.0	76.3
psbL_p5	CTTCCGAATGGGATAAGCGA	GGACTAGCTGTACCTACCGT	50.0	55.0	57.5	58.0	269 bp	1.0	76.0