Hotspot

trnN(GTT)-trnR(ACG)

IRa · 134824-135464 · 641 bp

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Pi 0.00473

MarkerSeek score 62.50

Primer available yes

Label trnN(GTT)-trnR(ACG)

Feature Metadata

label_name: trnN(GTT)-trnR(ACG)
feature_type: igs
region: IRa
length_bp: 641
variable_sites: 3
pi: 0.00473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(GTT)-trnR(ACG)_p1	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	739-746 bp	1.0	78.1
trnN(GTT)-trnR(ACG)_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	726-733 bp	1.0	78.1
trnN(GTT)-trnR(ACG)_p3	TAGAATCTCCCGCTGTTCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	784-791 bp	1.0	78.0
trnN(GTT)-trnR(ACG)_p4	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	727-734 bp	1.0	77.7
trnN(GTT)-trnR(ACG)_p5	GCTGTTAACTGACTGGTCGT	GAACAGATCCAGTGGAGACG	50.0	55.0	57.8	57.7	776-783 bp	1.0	77.5