Hotspot

ccsA-ndhD

SSC · 120260-120563 · 304 bp

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Pi 0.0468

MarkerSeek score 81.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 304
variable_sites: 14
pi: 0.0468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTAGCTTCCATGGGCTTTC	CAGTCGACAAGGTTGAAGCT	50.0	50.0	57.9	58.1	453-457 bp	1.0	81.3
ccsA-ndhD_p2	TGTAGCTTCCATGGGCTTTC	TCAGTCGACAAGGTTGAAGC	50.0	50.0	57.9	58.1	454-458 bp	1.0	81.2
ccsA-ndhD_p3	TTCCGCAATTGTAGCTTCCA	CAGTCGACAAGGTTGAAGCT	45.0	50.0	57.8	58.1	462-466 bp	1.0	80.7
ccsA-ndhD_p4	TTCCGCAATTGTAGCTTCCA	TCAGTCGACAAGGTTGAAGC	45.0	50.0	57.8	58.1	463-467 bp	1.0	80.7
ccsA-ndhD_p5	TCCGCAATTGTAGCTTCCAT	CAGTCGACAAGGTTGAAGCT	45.0	50.0	57.6	58.1	461-465 bp	1.0	78.9