Hotspot

ndhF-rpl32

SSC · 116846-117798 · 953 bp

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Pi 0.00846

MarkerSeek score 61.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 953
variable_sites: 8
pi: 0.00846
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	CTGCCCAATATCCCCGTTTT	40.0	50.0	57.0	58.2	1134-1135 bp	1.0	70.9
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	TGCCCAATATCCCCGTTTTT	40.0	45.0	57.0	57.8	1133-1134 bp	1.0	70.8
ndhF-rpl32_p3	TTTGTTGCCGTTGGAAAAAGT	CTGCCCAATATCCCCGTTTT	38.1	50.0	57.7	58.2	1135-1136 bp	1.0	68.7
ndhF-rpl32_p4	TTTGTTGCCGTTGGAAAAAGT	TGCCCAATATCCCCGTTTTT	38.1	45.0	57.7	57.8	1134-1135 bp	1.0	68.6
ndhF-rpl32_p5	GTTGCCGTTGGAAAAAGTACA	CTGCCCAATATCCCCGTTTT	42.9	50.0	57.6	58.2	1132-1133 bp	1.0	67.9