Hotspot

petA-psbJ

LSC · 65886-66637 · 752 bp

Back to Nephelium

Pi 0.0175

MarkerSeek score 59.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 13
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGATTGGGTTCGTCGTTGTA	50.0	50.0	58.0	57.9	892-902 bp	1.0	81.2
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	GGATTGGGTTCGTCGTTGTA	50.0	50.0	58.2	57.9	889-899 bp	1.0	80.1
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	GGATTGGGTTCGTCGTTGTA	45.0	50.0	57.8	57.9	857-867 bp	1.0	79.8
petA-psbJ_p4	TTGGCATCTGTTGTTTTGGC	GGATTGGGTTCGTCGTTGTA	45.0	50.0	57.8	57.9	860-870 bp	1.0	79.7
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	GGATTGGGTTCGTCGTTGTA	45.0	50.0	57.6	57.9	887-897 bp	1.0	78.7