Hotspot

ndhC-trnV(TAC)

LSC · 53329-54209 · 881 bp

Back to Nephelium

Pi 0.00577

MarkerSeek score 63.60

Primer available yes

Label ndhC-trnV(TAC)

Feature Metadata

label_name: ndhC-trnV(TAC)
feature_type: igs
region: LSC
length_bp: 881
variable_sites: 5
pi: 0.00577
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV(TAC)_p1	CTGGTCCTTTGCTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	1027-1029 bp	1.0	77.2
ndhC-trnV(TAC)_p2	TAATCGGGGCTAAAACTGCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1015-1017 bp	1.0	77.2
ndhC-trnV(TAC)_p3	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1022-1024 bp	1.0	77.2
ndhC-trnV(TAC)_p4	TCCTTTGCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	1023-1025 bp	1.0	75.9
ndhC-trnV(TAC)_p5	CTGGTCCTTTGCTAATCGGG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.3	57.5	1026-1028 bp	1.0	74.5