Hotspot

psaA-ycf3

LSC · 44322-45129 · 808 bp

Back to Nephelium

Pi 0.00397

MarkerSeek score 65.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 3
pi: 0.00397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	876-928 bp	1.0	82.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	868-920 bp	1.0	82.3
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTCGGAGGCTTGGTTCAATC	50.0	50.0	57.9	58.2	951-1003 bp	1.0	81.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	ACAAGCTATCACGCTCACTC	50.0	50.0	57.9	57.7	912-964 bp	1.0	81.0
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTTCGGAGGCTTGGTTCAAT	50.0	45.0	57.9	57.7	952-1004 bp	1.0	81.0