Hotspot

trnS(TGA)-psbZ

LSC · 37851-38201 · 351 bp

Back to Nephelium

Pi 0.00857
MarkerSeek score 52.00
Primer available yes
Label trnS(TGA)-psbZ

Feature Metadata

label_name
trnS(TGA)-psbZ
feature_type
igs
region
LSC
length_bp
351
variable_sites
3
pi
0.00857
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(TGA)-psbZ_p1 CAAGACCGGAGCTATCAACC CGACCAACCATCAGGAGAAG 55.0 55.0 58.1 58.0 489 bp 1.0 80.1
trnS(TGA)-psbZ_p2 CTATCAACCACTCGGCCATC CGACCAACCATCAGGAGAAG 55.0 55.0 58.1 58.0 478 bp 1.0 79.5
trnS(TGA)-psbZ_p3 TTCACCGAGCAAAAGGAGAG CGACCAACCATCAGGAGAAG 50.0 55.0 57.8 58.0 559 bp 1.0 79.1
trnS(TGA)-psbZ_p4 TTTGTTCCGAACCATACCGG CGACCAACCATCAGGAGAAG 50.0 55.0 58.2 58.0 513 bp 1.0 79.0
trnS(TGA)-psbZ_p5 CAAGACCGGAGCTATCAACC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.1 57.8 486 bp 1.0 78.5