Hotspot

trnT(GGT)-psbD

LSC · 33570-35088 · 1519 bp

Back to Nephelium

Pi 0.00922
MarkerSeek score 63.40
Primer available yes
Label trnT(GGT)-psbD

Feature Metadata

label_name
trnT(GGT)-psbD
feature_type
igs
region
LSC
length_bp
1519
variable_sites
14
pi
0.00922
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(GGT)-psbD_p1 GTGGTAGAGTAACGCCATGG TCTCCGTAACCAGTCATCCA 55.0 50.0 58.1 57.8 1652-1659 bp 1.0 78.4
trnT(GGT)-psbD_p2 TCAGTGGTAGAGTAACGCCA TCTCCGTAACCAGTCATCCA 50.0 50.0 58.1 57.8 1655-1662 bp 1.0 78.2
trnT(GGT)-psbD_p3 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1683-1690 bp 1.0 78.1
trnT(GGT)-psbD_p4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1686-1693 bp 1.0 77.9
trnT(GGT)-psbD_p5 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 55.0 50.0 58.1 57.7 1685-1692 bp 1.0 77.7