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psbM-trnD(GTC)

LSC · 30801-31974 · 1174 bp

Back to Nephelium

Pi 0.00852

MarkerSeek score 63.60

Primer available yes

Label psbM-trnD(GTC)

Feature Metadata

label_name: psbM-trnD(GTC)
feature_type: igs
region: LSC
length_bp: 1174
variable_sites: 10
pi: 0.00852
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD(GTC)_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1285-1297 bp	1.0	59.0
psbM-trnD(GTC)_p2	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	1292-1304 bp	1.0	55.8
psbM-trnD(GTC)_p3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	1286-1298 bp	1.0	54.8
psbM-trnD(GTC)_p4	GGAACTAGAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.1	58.5	1291-1303 bp	1.0	53.3
psbM-trnD(GTC)_p5	AGAATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	45.0	52.6	55.8	57.8	1284-1296 bp	1.0	52.9