Hotspot

petN-psbM

LSC · 29418-30695 · 1278 bp

Back to Nephelium

Pi 0.00548

MarkerSeek score 61.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1278
variable_sites: 7
pi: 0.00548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1440-1449 bp	1.0	67.9
petN-psbM_p2	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.8	57.6	1380-1389 bp	1.0	67.7
petN-psbM_p3	GGGGAAGGAGTGGACTCTAG	TGCTACTGCACTGTTCATTCT	60.0	42.9	58.2	57.6	1377-1386 bp	1.0	67.4
petN-psbM_p4	CTTGGGCTGCGTTAATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	1428-1437 bp	1.0	67.1
petN-psbM_p5	TTGGGCTGCGTTAATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	1427-1436 bp	1.0	67.1