Hotspot

rpoB-trnC(GCA)

LSC · 27455-28665 · 1211 bp

Back to Nephelium

Pi 0.005

MarkerSeek score 62.40

Primer available yes

Label rpoB-trnC(GCA)

Feature Metadata

label_name: rpoB-trnC(GCA)
feature_type: igs
region: LSC
length_bp: 1211
variable_sites: 6
pi: 0.005
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC(GCA)_p1	CCGGGGATTGTAGACATTCG	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.1	58.4	1286-1296 bp	1.0	77.1
rpoB-trnC(GCA)_p2	CCGGGGATTGTAGACATTCG	GCGACACCCAGATTTGAACT	55.0	50.0	58.1	58.5	1311-1321 bp	1.0	76.1
rpoB-trnC(GCA)_p3	CCCGGGGATTGTAGACATTC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.7	58.4	1287-1297 bp	1.0	76.1
rpoB-trnC(GCA)_p4	GGGGATTGTAGACATTCGCA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1284-1294 bp	1.0	75.5
rpoB-trnC(GCA)_p5	CCCGGGGATTGTAGACATTC	GCGACACCCAGATTTGAACT	55.0	50.0	57.7	58.5	1312-1322 bp	1.0	75.1