Hotspot

atpH-atpI

LSC · 14019-15221 · 1203 bp

Back to Nephelium

Pi 0.00748

MarkerSeek score 56.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 9
pi: 0.00748
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	AAGCGGCATTCAAGCTCTTA	50.0	45.0	58.0	57.9	1345-1350 bp	1.0	79.4
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1324-1329 bp	1.0	79.2
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	AAGCGGCATTCAAGCTCTTA	50.0	45.0	57.9	57.9	1367-1372 bp	1.0	78.7
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1346-1351 bp	1.0	78.6
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	AAGCGGCATTCAAGCTCTTA	55.0	45.0	58.2	57.9	1371-1376 bp	1.0	78.1