Hotspot

trnR(TCT)-atpA

LSC · 10190-10449 · 260 bp

Back to Nephelium

Pi 0.0286
MarkerSeek score 62.80
Primer available yes
Label trnR(TCT)-atpA

Feature Metadata

label_name
trnR(TCT)-atpA
feature_type
igs
region
LSC
length_bp
260
variable_sites
7
pi
0.0286
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR(TCT)-atpA_p1 TGCAAAGCGTCCATTGTCTA GGATTCAGGAACAAATGGAACG 45.0 45.5 57.8 57.9 370-385 bp 1.0 66.5
trnR(TCT)-atpA_p2 TGCAAAGCGTCCATTGTCTA AGGGATTCAGGAACAAATGGA 45.0 42.9 57.8 56.9 372-387 bp 1.0 66.3
trnR(TCT)-atpA_p3 AATTCGGAATGCAAAGCGTC GGATTCAGGAACAAATGGAACG 45.0 45.5 57.7 57.9 379-394 bp 1.0 65.7
trnR(TCT)-atpA_p4 AATTCGGAATGCAAAGCGTC AGGGATTCAGGAACAAATGGA 45.0 42.9 57.7 56.9 381-396 bp 1.0 65.5
trnR(TCT)-atpA_p5 AATGCAAAGCGTCCATTGTC GGATTCAGGAACAAATGGAACG 45.0 45.5 57.6 57.9 372-387 bp 1.0 65.0