Hotspot

psbA

LSC · 340-1398 · 1059 bp

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Pi 0.00396
MarkerSeek score 42.00
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1059
variable_sites
14
pi
0.00396
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 GATCCACTTGGCTACATCCG GAAAGCCGTGTGCAATGAAA 55.0 45.0 58.1 57.9 1682-1810 bp 0.857143 78.7
psbA_p2 GATCCACTTGGCTACATCCG CCTCCCCAAAAGTCACTTCC 55.0 55.0 58.1 58.1 1985-2085 bp 0.285714 52.4
psbA_p3 CAATCCACTGCCTTGATCCA CCTCCCCAAAAGTCACTTCC 50.0 55.0 57.9 58.1 1999-2099 bp 0.285714 52.4
psbA_p4 AATCCACTGCCTTGATCCAC CCTCCCCAAAAGTCACTTCC 50.0 55.0 57.9 58.1 1998-2098 bp 0.285714 52.4
psbA_p5 ACAATCCACTGCCTTGATCC CCTCCCCAAAAGTCACTTCC 50.0 55.0 57.9 58.1 2000-2100 bp 0.285714 52.4