Hotspot

ccsA-ndhD

SSC · 113043-113295 · 253 bp

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Pi 0.0468
MarkerSeek score 76.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
253
variable_sites
33
pi
0.0468
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTGGAGTTGGGACCCAAAAG CTCGCATTACCGGGTATGAG 50.0 55.0 57.9 57.9 808-820 bp 1.0 87.1
ccsA-ndhD_p2 TTGGAGTTGGGACCCAAAAG GGTTGATAATAGTGGGCGCA 50.0 50.0 57.9 58.0 1128-1140 bp 0.857143 79.5
ccsA-ndhD_p3 TTGGAGTTGGGACCCAAAAG TGGTTGATAATAGTGGGCGC 50.0 50.0 57.9 58.0 1129-1141 bp 0.857143 79.5
ccsA-ndhD_p4 CTAACGAAGCATGGGGATCC GGTTGATAATAGTGGGCGCA 55.0 50.0 58.1 58.0 1150-1162 bp 0.857143 79.1
ccsA-ndhD_p5 CTAACGAAGCATGGGGATCC TGGTTGATAATAGTGGGCGC 55.0 50.0 58.1 58.0 1151-1163 bp 0.857143 79.1