Hotspot

rps18-rpl20

LSC · 67345-67569 · 225 bp

Back to Nepeta

Pi 0.0635
MarkerSeek score 72.00
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
225
variable_sites
26
pi
0.0635
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 AAAGGAGGTTCATGGCCAAG TCGTCGTTTGTGGATCACTC 50.0 50.0 58.1 57.9 1131-1142 bp 1.0 84.6
rps18-rpl20_p2 AAAGGAGGTTCATGGCCAAG CGTTTGTGGATCACTCGGAT 50.0 50.0 58.1 58.0 1127-1138 bp 0.857143 76.9
rps18-rpl20_p3 ATCCAAAGGAGGTTCATGGC CGTTTGTGGATCACTCGGAT 50.0 50.0 57.9 58.0 1131-1142 bp 0.857143 76.3
rps18-rpl20_p4 TGAAAGGGGGAAAAATGCCA CGTTTGTGGATCACTCGGAT 45.0 50.0 57.9 58.0 891-902 bp 0.714286 70.2
rps18-rpl20_p5 TGAAAGGGGGAAAAATGCCA TCGTCGTTTGTGGATCACTC 45.0 50.0 57.9 57.9 895-906 bp 0.714286 69.8