Hotspot

petA-psbJ

LSC · 62255-63291 · 1037 bp

Back to Nepeta

Pi 0.0347
MarkerSeek score 78.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1037
variable_sites
98
pi
0.0347
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA ATTCCGGATTAGGTTCGTCC 50.0 50.0 58.2 57.1 987-1114 bp 1.0 82.6
petA-psbJ_p2 AGAAGGTTCAATTGGCCGAA ATTCCGGATTAGGTTCGTCC 45.0 50.0 57.7 57.1 986-1113 bp 1.0 81.8
petA-psbJ_p3 GAGAAGGTTCAATTGGCCGA TTCCGGATTAGGTTCGTCCC 50.0 55.0 58.2 59.2 986-1113 bp 1.0 80.5
petA-psbJ_p4 GAGAAGGTTCAATTGGCCGA CGGATTAGGTTCGTCCCTGT 50.0 55.0 58.2 59.2 983-1110 bp 1.0 80.5
petA-psbJ_p5 AGAAGGTTCAATTGGCCGAA TTCCGGATTAGGTTCGTCCC 45.0 55.0 57.7 59.2 985-1112 bp 1.0 79.6