Hotspot

trnG-GCC-trnM-CAU

LSC · 35520-35689 · 170 bp

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Pi 0.0381
MarkerSeek score 71.60
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
170
variable_sites
14
pi
0.0381
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 AGAAGATGCGGGTTCGATTC GTTCAAATCCTGTCTCCGCA 50.0 50.0 58.1 58.2 214-229 bp 1.0 87.1
trnG-GCC-trnM-CAU_p2 GAGAAGATGCGGGTTCGATT GTTCAAATCCTGTCTCCGCA 50.0 50.0 58.1 58.2 215-230 bp 1.0 87.1
trnG-GCC-trnM-CAU_p3 AGAAGATGCGGGTTCGATTC TTCAAATCCTGTCTCCGCAA 50.0 45.0 58.1 57.4 213-228 bp 1.0 84.2
trnG-GCC-trnM-CAU_p4 GAGAAGATGCGGGTTCGATT TTCAAATCCTGTCTCCGCAA 50.0 45.0 58.1 57.4 214-229 bp 1.0 84.1
trnG-GCC-trnM-CAU_p5 CAAGGAGAAGATGCGGGTTC GTTCAAATCCTGTCTCCGCA 55.0 50.0 58.6 58.2 219-234 bp 1.0 82.4