Hotspot

trnE-UUC-trnT-GGU

LSC · 29828-30393 · 566 bp

Back to Nepeta

Pi 0.0258
MarkerSeek score 76.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
566
variable_sites
46
pi
0.0258
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 679-707 bp 1.0 88.0
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 658-686 bp 1.0 87.7
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 655-683 bp 1.0 87.5
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 641-669 bp 1.0 87.4
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 55.0 55.0 57.8 58.2 676-704 bp 1.0 86.8